Reduced Cas9 transgene silencing by incorporation of intron sequences

Reduced Cas9 Transgene Silencing by Incorporation of Intron Sequences

Background

Silencing continues to pose a serious obstacle for stable expression of foreign genes in host organisms. Such silencing restricts both the penetrance and expressivity of transgenes, thereby reducing the efficiency of genetic engineering and related experimental systems.

Research Focus

This study explores ways to minimize Cas9 transgene silencing through the inclusion of intron sequences within the construct. By incorporating intronic elements, researchers aimed to promote proper transcriptional activity and maintain consistent Cas9 expression across cells.

Findings

The data indicate that the addition of selected introns lowers the risk of transcriptional silencing, resulting in improved gene activity and more uniform Cas9 expression. These results suggest intron design can serve as a practical strategy for strengthening transgene reliability in molecular biology applications.

“Silencing remains a significant challenge for exogenous gene expression, limiting both the penetrance and expressivity of transgenes.”

Implications

Enhanced transgene stability via intron incorporation may advance CRISPR-based genome editing, gene therapy, and transgenic model development. It contributes to more predictable gene regulation in research and applied biotechnology.

Author’s Summary: Introducing targeted intron sequences into Cas9 constructs reduces transgene silencing and ensures more consistent gene expression across host systems.

Nature — 2025-11-27